國家衛生研究院 NHRI:Item 3990099045/12653
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/12653


    Title: Generation and characterization of single chain variable fragment against alpha-enolase of Candida albicans
    Other Titles: Generation and characterization of single chain variable fragment against α-enolase of Candida albicans
    Authors: Leu, SJ;Lee, YC;Lee, CH;Liao, PY;Chiang, CW;Yang, CM;Su, CH;Ou, TY;Liu, KJ;Lo, HJ;Tsai, BY;Yang, YY
    Contributors: National Institute of Cancer Research;National Institute of Infectious Diseases and Vaccinology
    Abstract: Candida albicans (C. albicans) is an opportunistic human pathogen responsible for approximately a half of clinical candidemia. The emerging Candida spp. with resistance to azoles is a major challenge in clinic, suggesting an urgent demand for new drugs and therapeutic strategies. Alpha-enolase (Eno1) is a multifunctional protein and represents an important marker for invasive candidiasis. Thus, C. albicans Eno1 (CaEno1) is believed to be an important target for the development of therapeutic agents and antibody drugs. Recombinant CaEno1 (rCaEno1) was first used to immunize chickens. Subsequently, we used phage display technology to construct two single chain variable fragment (scFv) antibody libraries. A novel biopanning procedure was carried out to screen anti-rCaEno1 scFv antibodies, whose specificities were further characterized. The polyclonal IgY antibodies showed binding to rCaEno1 and native CaEno1. A dominant scFv (CaS1) and its properties were further characterized. CaS1 attenuated the growth of C. albicans and inhibited the binding of CaEno1 to plasminogen. Animal studies showed that CaS1 prolonged the survival rate of mice and zebrafish with candidiasis. The fungal burden in kidney and spleen, as well as level of inflammatory cytokines were significantly reduced in CaS1-treated mice. These results suggest CaS1 has potential of being immunotherapeutic drug against C. albicans infections.
    Date: 2020-04-21
    Relation: International Journal of Molecular Sciences. 2020 Apr 21;21(8):Article number 2903.
    Link to: http://dx.doi.org/10.3390/ijms21082903
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1422-0067&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000535565300245
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85084010098
    Appears in Collections:[Ko-Jiunn Liu] Periodical Articles
    [Hsiu-Jung Lo] Periodical Articles

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