國家衛生研究院 NHRI:Item 3990099045/1260

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Title:	Development and validation of a liquid chromatography-tandem mass spectrometry for the determination of BPR0L075, a novel antimicrotuble agent, in rat plasma: Application to a pharmacokinetic study
Authors:	Chang, YW;Chen, WC;Lin, KT;Chang, L;Yao, HT;Hsieh, HP;Lan, SJ;Chen, CT;Chao, YS;Yeh, TK
Contributors:	Division of Biotechnology and Pharmaceutical Research
Abstract:	A rapid and sensitive liquid chromatography-tandem mass spectrometric method (LC-MS/MS) had been developed and validated to determine the concentrations of BPROL075 in rat plasma. After a simple protein precipitation of plasma samples by acetonitrile, BPROL075 was analyzed on a C-8 column at a flow rate of 0.5 mL/min. The mobile phase consisted of a mixture of 10 mM: ammonium acetate containing 0.1% formic acid and acetonitrile (20:80, v/v). Both BPROL075 (analyte) and the internal standard (BPR0L092) were determined using electro-spray ionization and the MS data acquisition was via multiple reactions monitoring (MRM) in positive scanning model. The MS/MS ion transitions monitored are m/z 342.2/195.2 and 312.5/165.2 for BPROL075 and BPROL092, respectively. The low limit of quantitation was 0.5 ng/mL. Each plasma sample was chromatographed within 5 min. The method was validated with respect to linearity, accuracy, precision, recovery, and stability. A good linear relationship was observed over the concentration range of 0.5-1000 ng/mL (r > 0.9994). Absolute recoveries ranged from 63.45 to 68.34% in plasma at the concentrations of 2, 40, 400, and 800 ng/mL. The intra- and inter-day accuracy ranged from 92.04 to 111.80%. Intra- and inter-day relative standard deviations were 1.08-3.29% and 1.96-5.46%, respectively. This developed and validated assay method had been successfully applied to a pharmacokinetic study after intravenous injection of BPROL075 in rats at a dose of 5 mg/kg.
Keywords:	Biochemical Research Methods;Chemistry, Analytical
Date:	2007-02-01
Relation:	Journal of Chromatography B. 2007 Feb;846(1-2):162-168.
Link to:	http://dx.doi.org/10.1016/j.jchromb.2006.08.040
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1570-0232&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000244281100022
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33846501675
Appears in Collections:	[Hsing-Pang Hsieh] Periodical Articles [Teng-Kuang Yeh] Periodical Articles [Yu-Sheng Chao(2002-2013)] Periodical Articles [Chiung-Tong Chen] Periodical Articles

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