國家衛生研究院 NHRI:Item 3990099045/1255
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    题名: Shear stress inhibits smooth muscle cell-induced inflammatory gene expression in endothelial cells - Role of NF-kappa B
    作者: Chiu, JJ;Chang, SF;Lee, PL;Lee, CI;Tsai, MC;Lee, DY;Hsieh, HP;Usami, S;Chien, S
    贡献者: Division of Biotechnology and Pharmaceutical Research;Division of Medical Engineering Research
    摘要: Objectives-Vascular endothelial cells (ECs) are influenced by shear stress and neighboring smooth muscle cells (SMCs). We investigated the inflammation-relevant gene expression in EC/SMC cocultures under static condition and in response to shear stress. Materials and Methods-Under static condition, DNA microarrays and reverse-transcription polymerase chain reaction identified 23 inflammation-relevant genes in ECs whose expression was significantly affected by coculture with SMCs, with 18 upregulated and 5 downregulated. Application of shear stress (12 dynes/cm(2)) to the EC side of the coculture for 6 hours inhibited most of the proinflammatory gene expressions in ECs induced by coculture with SMCs. Inhibition of nuclear factor-κ B (NF-κ B) activation by the p65-antisense, lactacystin, and N-acetyl-cysteine blocked the coculture-induced EC expression of proinflammatory genes, indicating that the NF-κ B binding sites in the promoters of these genes play a significant role in their expression as a result of coculture with SMCs. Chromatin immunoprecipitation assays demonstrated the in vivo regulation of NF-κ B recruitment to selected target promoters. Shear stress inhibited the SMC coculture-induced NF-κ B activation in ECs and monocytic THP-1 cell adhesion to ECs. Conclusions-Our findings suggest that shear stress plays an inhibitory role in the proinflammatory gene expression in ECs located in close proximity to SMCs.
    关键词: Hematology;Peripheral Vascular Disease
    日期: 2005-05
    關聯: Arteriosclerosis, Thrombosis, and Vascular Biology. 2005 May;25(5):963-969.
    Link to: http://dx.doi.org/10.1161/01.ATV.0000159703.43374.19
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1079-5642&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000228806900371
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=18244378805
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