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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/12548


    Title: Identification and engineering of 32 membered antifungal macrolactone notonesomycins
    Authors: Goh, F;Zhang, MM;Lim, TR;Low, KN;Nge, CE;Heng, E;Yeo, WL;Sirota, FL;Crasta, S;Tan, Z;Ng, V;Leong, CY;Zhang, H;Lezhava, A;Chen, SL;Hoon, SS;Eisenhaber, F;Eisenhaber, B;Kanagasundaram, Y;Wong, FT;Ng, SB
    Contributors: Institute of Molecular and Genomic Medicine
    Abstract: Notonesomycin A is a 32-membered bioactive glycosylated macrolactone known to be produced by Streptomyces aminophilus subsp. notonesogenes 647-AV1 and S. aminophilus DSM 40186. In a high throughput antifungal screening campaign, we identified an alternative notonesomycin A producing strain, Streptomyces sp. A793, and its biosynthetic gene cluster. From this strain, we further characterized a new more potent antifungal non-sulfated analogue, named notonesomycin B. Through CRISPR-Cas9 engineering of the biosynthetic gene cluster, we were able to increase the production yield of notonesomycin B by up to 18-fold as well as generate a strain that exclusively produces this analogue.
    Date: 2020-03-19
    Relation: Microbial Cell Factories. 2020 Mar 19;19:Article number 71.
    Link to: http://dx.doi.org/10.1186/s12934-020-01328-x
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1475-2859&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000522109500001
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85082054693
    Appears in Collections:[張明姿] 期刊論文

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