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Please use this identifier to cite or link to this item:
http://ir.nhri.org.tw/handle/3990099045/12548
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Title: | Identification and engineering of 32 membered antifungal macrolactone notonesomycins |
Authors: | Goh, F;Zhang, MM;Lim, TR;Low, KN;Nge, CE;Heng, E;Yeo, WL;Sirota, FL;Crasta, S;Tan, Z;Ng, V;Leong, CY;Zhang, H;Lezhava, A;Chen, SL;Hoon, SS;Eisenhaber, F;Eisenhaber, B;Kanagasundaram, Y;Wong, FT;Ng, SB |
Contributors: | Institute of Molecular and Genomic Medicine |
Abstract: | Notonesomycin A is a 32-membered bioactive glycosylated macrolactone known to be produced by Streptomyces aminophilus subsp. notonesogenes 647-AV1 and S. aminophilus DSM 40186. In a high throughput antifungal screening campaign, we identified an alternative notonesomycin A producing strain, Streptomyces sp. A793, and its biosynthetic gene cluster. From this strain, we further characterized a new more potent antifungal non-sulfated analogue, named notonesomycin B. Through CRISPR-Cas9 engineering of the biosynthetic gene cluster, we were able to increase the production yield of notonesomycin B by up to 18-fold as well as generate a strain that exclusively produces this analogue. |
Date: | 2020-03-19 |
Relation: | Microbial Cell Factories. 2020 Mar 19;19:Article number 71. |
Link to: | http://dx.doi.org/10.1186/s12934-020-01328-x |
JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1475-2859&DestApp=IC2JCR |
Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000522109500001 |
Cited Times(Scopus): | https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85082054693 |
Appears in Collections: | [張明姿] 期刊論文
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