國家衛生研究院 NHRI:Item 3990099045/12475
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    題名: Human milk Oligosaccharide 2 '-Fucosyllactose reduces neurodegeneration in stroke brain
    作者: Wu, KJ;Chen, YH;Bae, EK;Song, Y;Min, W;Yu, SJ
    貢獻者: Center for Neuropsychiatric Research
    摘要: 2 '-Fucosyllactose (2'-FL) is a major oligosaccharide in human milk and is present at trace levels in cow milk. 2'-FL reduces inflammation in the gastrointestinal tract. Its action in the central nervous system has not been well characterized. The purpose of this study is to determine 2'-FL-mediated neural protection and repair in culture and stroke brain. In rat primary cortical neuronal cultures, 2'-FL significantly antagonized N-methyl-D-aspartate (NMDA) or glutamate-mediated changes in ATP production, MAP2 immunoreactivity, and TUNEL. The influx of Ca++ (Ca(++)i) was examined in primary cortical neurons expressing GCaMP5, an endogenous calcium probe. NMDA increased Ca(++)i; 2'-FL significantly attenuated this reaction. In a rat middle cerebral artery occlusion model of stroke, we found that intracerebroventricular pretreatment or oral posttreatment with 2'-FL significantly reduced brain infarction, mitigated microglial activation, improved locomotor activity, and upregulated brain-derived neurotrophic factor (BDNF) expression. Post-stroke delivery of 2'-FL increased bromodeoxyuridine (BrdU) labeling in the perilesioned area. These BrdU cells co-expressed NeuN, or nestin, or GFAP. Using subventricular Matrigel cultures, we demonstrated that 2'-FL increased cell migration from subventricular zone explant. This response was reduced by anti-BDNF blocking antibody. In conclusion, our data suggest that 2'-FL has neuroprotective action through inhibition of Ca(++)i, inflammation, and apoptosis. Posttreatment with 2'-FL facilitates neural repair in stroke brain.
    日期: 2020-10
    關聯: Translational Stroke Research. 2020 Oct;11:1001-1011.
    Link to: http://dx.doi.org/10.1007/s12975-019-00774-z
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1868-4483&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000505374600002
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85077256320
    顯示於類別:[劉誠珍] 期刊論文

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