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http://ir.nhri.org.tw/handle/3990099045/12418
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| Title: | ERK activation modulates cancer stemness and motility of a novel mouse oral squamous cell carcinoma cell line |
| Authors: | Chen, YL;Liu, KJ;Jang, CW;Hsu, CC;Yen, YC;Liu, YL;Chuang, TH;Wang, SH;Fu, YK;Kuo, CC;Chen, YW |
| Contributors: | Institute of Biotechnology and Pharmaceutical Research;National Institute of Cancer Research;Immunology Research Center |
| Abstract: | We established the NHRI-HN1 cell line from a mouse tongue tumor induced by 4-nitroquinoline 1-oxide (4-NQO)/arecoline, with further selection for cell stemness via in vitro sphere culture, to evaluate potential immunotherapies for oral squamous cell carcinoma (OSCC) in East and Southeast Asia. In vivo and in vitro phenotypic characterization, including tumor growth, immune modulator administration, gene expression, morphology, migration, invasion, and sphere formation assays, were conducted. NHRI-HN1 cells are capable of generating orthotopic tumors in syngeneic mice. Interestingly, immune stimulation via CpG oligodeoxynucleotide (CpG-ODN) dramatically reduced the tumor growth in NHRI-HN1 cell-injected syngeneic mice. The pathways enriched in genes that were differentially expressed in NHRI-HN1 cells when compared to non-tumorigenic cells were similar to those that were identified when comparing human OSCC and non-tumorous tissues. NHRI-HN1 cells have characteristics of epithelial-mesenchymal transition (EMT), including enhanced migration and invasion. NHRI-HN1 cells showed aggressive cell growth and sphere formation. The blockage of extracellular signal-regulated kinase (ERK) activation suppressed cell migration and reduced stemness characteristics in NHRI-HN1 cells, similar to human OSCC cell lines. Our data suggest that NHRI-HN1 cells, showing tumorigenic characteristics of EMT, cancer stemness, and ERK activation, are sufficient in modeling human OSCC and also competent for use in investigating oral cancer immunotherapies. |
| Date: | 2019-12-24 |
| Relation: | Cancers. 2019 Dec 24;12(1):Article number 61. |
| Link to: | http://dx.doi.org/10.3390/cancers12010061 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2072-6694&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000516826700061 |
| Cited Times(Scopus): | https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85077310837 |
| Appears in Collections: | [郭靜娟] 期刊論文
[陳雅雯] 期刊論文
[劉柯俊] 期刊論文
[莊宗顯] 期刊論文
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| PUB31878324.pdf | | 21658Kb | Adobe PDF | 441 | View/Open |
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