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Please use this identifier to cite or link to this item:
http://ir.nhri.org.tw/handle/3990099045/1237
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| Title: | Dipeptidyl peptidase 8/9-like activity in human leukocytes |
| Authors: | Maes, MB;Dubois, V;Brandt, I;Lambeir, AM;Van der Veken, P;Augustyns, K;Cheng, JD;Chen, X;Scharpe, S;De Meester, I |
| Contributors: | Division of Biotechnology and Pharmaceutical Research |
| Abstract: | The proline-specific dipeptidyl peptidases (DPPs) are emerging as a protease family with important roles in the regulation of signaling by peptide hormones. Inhibitors of DPPs have an intriguing, therapeutic potential, with clinical efficacy seen in patients with diabetes. Until now, only recombinant forms of DPP8 and DPP9 have been characterized. Their enzymatic activities have not been demonstrated in or purified from any natural source. Using several selective DPP inhibitors, we show that DPP activity, attributable to DPP8/9 is present in human PBMC. All leukocyte types tested (lymphocytes, monocytes, Jurkat, and U937 cells) were shown to contain similar DPP8/9-specific activities, and DPPII- and DPPIV-specific activities varied considerably. The results were confirmed by DPPIV/CD26 immunocapture experiments. Subcellular fractionation localized the preponderance of DPP8/9 activity to the cytosol and DPPIV in the membrane fractions. Using Jurkat cell cytosol as a source., a 30-fold, enriched DPP preparation was obtained, which had enzymatic characteristics closely related to the ones of DPP8 and/or -9, including inhibition by allo-Re-isoindoline and affinity for immobilized Lys-isoindoline. |
| Keywords: | Cell Biology;Hematology;Immunology |
| Date: | 2007-05 |
| Relation: | Journal of Leukocyte Biology. 2007 May;81(5):1252-1257. |
| Link to: | http://dx.doi.org/10.1189/jlb.0906546 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0741-5400&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000246121800013 |
| Cited Times(Scopus): | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34247898818 |
| Appears in Collections: | [陳新(2002-2015)] 期刊論文
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| 000246121800013.pdf | | 263Kb | Adobe PDF | 547 | View/Open |
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