國家衛生研究院 NHRI:Item 3990099045/1229

NHRI > Institute of Biotechnology and Pharmaceutical Research > Xin Chen(2002-2015) > Periodical Articles > Item 3990099045/1229

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/1229

Title:	Purification and characterization of human prolyl dipeptidase DPPS in Sf9 insect cells
Authors:	Chen, YS;Chien, CH;Goparaju, CM;Hsu, JTA;Liang, PH;Chen, X
Contributors:	Division of Biotechnology and Pharmaceutical Research
Abstract:	DPP8 is a new member of the prolyl dipeptidases, many of which have important biological functions in vivo. DPP8 catalyzes the cleavage at the carboxyl side of the proline residue at the penultimate position. To study its structure and biochemical properties, we have overexpressed the human DPP8 protein in baculovirus infected Sf9 cells. The protein is soluble and can be purified to homogeneity. Using the chromogenic H-Gly-Pro-pNA as the substrate, a kinetic study shows that purified DPP8 is active and has a similar k(cat) value as that of DPP-IV, a prolyl dipeptidase that is a drug target for type II diabetes. The kinetic constants of DPP8 are also determined for other chromogenic substrates, and the results indicate that DPP8 has substrate preference at both the P1 and P2 sites. The expression system provides means of better understanding the structure, catalytic mechanism, and biological function of DPP8 protein. (C) 2004 Elsevier Inc. All rights reserved.
Keywords:	Biochemical Research Methods;Biochemistry & Molecular Biology;Biotechnology & Applied Microbiology
Date:	2004-05
Relation:	Protein Expression and Purification. 2004 May;35(1):142-146.
Link to:	http://dx.doi.org/10.1016/j.pep.2003.12.019
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1046-5928&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000220617600019
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=10644284275
Appears in Collections:	[Xin Chen(2002-2015)] Periodical Articles [John Tsu-An Hsu] Periodical Articles

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