國家衛生研究院 NHRI:Item 3990099045/12095

NHRI > Institute of Biomedical Engineering and Nanomedicine > Chia-Hsien Hsu > Periodical Articles > Item 3990099045/12095

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/12095

Title:	Peanut detection using droplet microfluidic polymerase chain reaction device
Authors:	Ma, SY;Chiang, YC;Hsu, CH;Chen, JJ;Hsu, CC;Chao, AC;Lin, YS
Contributors:	Institute of Biomedical Engineering and Nanomedicine
Abstract:	In this study, we integrated genetic detection for polymerase chain reaction (PCR) with microfluidics technology for the detection of peanut DNA. A cross-junction microchannel was used to induce emulsion droplets of water in oil for PCR on a chip. Compared with the single-phase flow, the emulsion droplet flow exhibited a 7.24% lower evaporation amount and prevented air bubble generation. PCR results of the droplet microfluidic PCR chip for peanut DNA fragment detection was verified by comparison with a commercial PCR thermal cycler and increased fluorescence intensity in SYBR Green reagent-based PCR. Moreover, PCR on the microfluidic PCR chip was successful for sesame, Salmonella spp., and Staphylococcus aureus. The droplet microfluidic PCR device developed in this study can be applied for peanut detection in the context of food allergy.
Date:	2019-05-02
Relation:	Journal of Sensors. 2019 May 2;2019:Article number 4712084.
Link to:	http://dx.doi.org/10.1155/2019/4712084
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1687-725X&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000473429300001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85073890586
Appears in Collections:	[Chia-Hsien Hsu] Periodical Articles

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