The serine/threonine kinase GCK-like kinase (GLK) is first identified by screening a human skeletal muscle cDNA library using degenerate oligonucleotide PCR primers (Diener et al. 1997). The nucleotide sequence of GLK encodes an open reading frame of 894 amino acids with a calculated molecular mass of 100 kDa. GLK shares 57% amino acid identity with MAP4K2 (also named germinal center kinase; GCK) and 49% amino acid identity with MAP4K1 (also named hematopoietic progenitor kinase 1 (HPK1)) (Hu et al. 1996; Diener et al. 1997). Thus, GLK is classified as a member of the MAP kinase kinase kinase kinase (MAP4K) family and also named MAP4K3. The MAP4K3/GLK protein contains a conserved N-terminal kinase domain, three proline-rich regions, and a conserved C-terminal citron homology domain (Fig. 1). Three splice variants of...
Date:
2018
Relation:
Encyclopedia of Signaling Molecules. 2018 :2947-2951.
