國家衛生研究院 NHRI:Item 3990099045/11950

NHRI > Institute of Biomedical Engineering and Nanomedicine > Chia-Hsien Hsu > Periodical Articles > Item 3990099045/11950

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/11950

Title:	Multilayer architecture microfluidic network array for combinatorial drug testing on 3D-cultured cells
Authors:	Chang, HC;Lin, CH;Juang, DS;Wu, HW;Lee, CY;Chen, C;Hsu, CH
Contributors:	Institute of Biomedical Engineering and Nanomedicine
Abstract:	In vitro testing of drug compounds on cell models during the drug development process represents an indispensable step in the initial screening process. Although drug testing on 3D cultured cells may provide a more accurate prediction of drug efficacy, it is relatively costly and time-consuming to perform compared with conventional 2D cultures due to the thick z-axis of the 3D models. In this study, we have presented a microfluidic platform with integrated pneumatic valves for producing a thin-gel 3D cell culture-based combinatorial drug screening array (3D-microCDS array). The multilayer architecture and microfluidic layout has a smaller device footprint than a single-layer microfluidic channel arrangement, making it well suited to scaling up for high-throughput combinatorial drug screening on 3D cell model. We performed 8 x 8 combination drug screening experiments with the device using two anti-cancer drugs (doxorubicin and paclitaxel) on MDA-MB-231 and MCF-7 breast cancer cell lines for demonstration. Our results indicate that our 3D-microCDS array device allows the successful screening of multiple drug combinations while reducing the operation time and the number of sample/reagents required, making it an ideal tool for general combinatorial drug screening, as well as for applications using valuable tissues and clinical samples.
Date:	2019-06
Relation:	Biofabrication. 2019 Jun;11(3):Article number 035024.
Link to:	http://dx.doi.org/10.1088/1758-5090/ab1f52
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1758-5082&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000470752400001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85067373727
Appears in Collections:	[Chia-Hsien Hsu] Periodical Articles

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