國家衛生研究院 NHRI:Item 3990099045/11898
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 12189/12972 (94%)
造訪人次 : 966379      線上人數 : 821
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於NHRI ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/11898


    題名: Sun1 mediates interkinetic nuclear migration and Notch signaling in the neurogenesis of zebrafish
    作者: You, MS;Wang, WP;Wang, JY;Jiang, YJ;Chi, YH
    貢獻者: Institute of Biotechnology and Pharmaceutical Research;Institute of Molecular and Genomic Medicine
    摘要: Interkinetic nuclear migration (INM) is a process by which nuclei oscillate between the basal and apical surfaces of epithelial cells in coordination with the cell cycle. The cytoskeletal machinery including microtubules and actin have been reported to drive apical INM, however, the role of nuclear proteins in this process has yet to be fully elucidated. Here we investigated the function of a SUN-domain protein, Sun1, in zebrafish. We found that zebrafish Sun1 is highly expressed in the ventricular zone of the brain. Knocking down sun1 with an antisense morpholino oligonucleotides reduced the abundance of nestin- and gfap- expressing neural stem cells and progenitor cells. The live-cell imaging results showed that sun1 morphant cells migrated toward the basal side during the S phase, but failed to migrate apically during the G2 phase. On the other hand, the passive stochastic movement during the G2 phase was unaffected. Furthermore, down-regulating sun1 was shown to reduce the expression of genes associated with the Notch pathway, whereas the expression of genes in the Wnt pathway was less perturbed. Findings from this research suggest that the Sun1-mediated nucleo-cytoskeletal interaction contributes to apical nuclear migration, and may thus affect exposure to Notch signal, thereby altering the composition of the progenitor pool, in the embryonic neurogenesis of zebrafish.
    日期: 2019-08-15
    關聯: Stem Cells and Development. 2019 Aug 15;28(16):1116-1127.
    Link to: http://dx.doi.org/10.1089/scd.2019.0063
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1547-3287&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000478898000005
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85070744877
    顯示於類別:[紀雅惠] 期刊論文
    [江運金] 期刊論文

    文件中的檔案:

    檔案 大小格式瀏覽次數
    PUB31140357.pdf1491KbAdobe PDF375檢視/開啟


    在NHRI中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋