國家衛生研究院 NHRI:Item 3990099045/11846
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    题名: Imaging through the Whole Brain of Drosophila at lambda/20 Super-resolution
    作者: Lin, HY;Chu, LA;Yang, H;Hsu, KJ;Lin, YY;Lin, KH;Chu, SW;Chiang, AS
    贡献者: Institute of Molecular and Genomic Medicine
    摘要: Recently, many super-resolution technologies have been demonstrated, significantly affecting biological studies by observation of cellular structures down to nanometer precision. However, current super-resolution techniques mostly rely on wavefront engineering or wide-field imaging of signal blinking or fluctuation, and thus imaging depths are limited due to tissue scattering or aberration. Here we present a technique that is capable of imaging through an intact Drosophila brain with 20-nm lateral resolution at approximately 200 mum depth. The spatial resolution is provided by molecular localization of a photoconvertible fluorescent protein Kaede, whose red form is found to exhibit blinking state. The deep-tissue observation is enabled by optical sectioning of spinning disk microscopy, as well as reduced scattering from optical clearing. Together these techniques are readily available for many biologists, providing three-dimensional resolution of densely entangled dendritic fibers in a complete Drosophila brain. The method paves the way toward whole-brain neural network studies and is applicable to other high-resolution bioimaging.
    日期: 2019-03-28
    關聯: iScience. 2019 Mar 28;14:164-170.
    Link to: http://dx.doi.org/10.1016/j.isci.2019.03.025
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2589-0042&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000466145400014
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85066263853
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