國家衛生研究院 NHRI:Item 3990099045/1166
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    题名: Sf-Caspase-I-repressed stable cells: resistance to apoptosis and augmentation of recombinant protein production
    作者: Lin, CC;Hsu, JTA;Huang, KL;Tang, HK;Shu, CW;Lai, YK
    贡献者: Division of Biotechnology and Pharmaceutical Research
    摘要: Sf-Caspase-I [Spodoptera frugiperda (fall armyworm) caspase-1] is the most studied effector caspase of Lepidoptera and its activation may lead cells to apoptosis (programmed cell death) when under UV irradiation or baculovirus infection. In the present study, we repressed the expression of Sf-caspase-I in Sf9 (S. frugiperda 9) cells using constitutive RNA interference, and evaluated the effects of stress responses and the production of proteins in recombinant baculovirus-infected cells. The Sf-caspase-I-repressed stable cells, Sf9/pIBdsCasp-I and Sf9/pIBdsCasp-2, showed a significant increase in resistance to UV- and baculovirus-induced apoptosis. These cells produced higher levels of both intracellular (luciferase) and extracellular [SEAP (secreted alkaline phosphatase)] recombinant proteins than the parental cells when infected with recombinant baculovirus. Thus Sfcaspase-I-repressed stable cells have a greater ability to adapt to various culture conditions, and also to provide the benefits of high-level protein production.
    关键词: Biochemistry & Molecular Biology;Biotechnology & Applied Microbiology
    日期: 2007-09
    關聯: Biotechnology and Applied Biochemistry. 2007 Sep;48(Pt. 1):11-19.
    Link to: http://dx.doi.org/10.1042/BA20070044
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0885-4513&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000249605300002
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34548536184
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