國家衛生研究院 NHRI:Item 3990099045/1162

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Title:	Inhibition of hepatitis C virus replication by antimonial compounds
Authors:	Hwang, DR;Lin, RK;Leu, GZ;Lin, TY;Lien, TW;Yu, MC;Yeh, CT;Hsu, JTA
Contributors:	Division of Biotechnology and Pharmaceutical Research
Abstract:	Chronic hepatitis C virus (HCV) infection is a worldwide health problem causing serious complications, such as liver cirrhosis and hepatoma. Alpha interferon (IFN-alpha) or its polyethylene glycol-modified form combined with ribavirin is the only recommended therapy. However, an alternative therapy is needed due to the unsatisfactory cure rate of the IFN-based therapy. Using a modified reporter assay based on the HCV subgenomic-replicon system, we found that sodium stibogluconate (SSG), a compound used for leishmania treatment, suppressed HCV replication. We have previously reported that SSG is effective at inhibiting HCV replication in a cell line permissive for HCV infection/replication and in an ex vivo assay using fresh human liver slices obtained from patients infected with HCV (26). In this study, we show that the SSG 50% inhibitory dose for HCV replication is 0.2 to 0.3 mg/ml (equivalent to 345 to 517 mu M of Sb) in the HCV subgenomic-replicon system. We also found that SSG and IFN-alpha exert a strong synergistic anti-HCV effect in both the traditional isobologram analysis and the median effect principle (CalcuSyn analysis). The combination of SSG and IFN-alpha could sustain the antiviral response better than SSG or IFN-alpha alone. The results suggest that SSG may be a good drug candidate for use in combination with other therapeutics, such as IFN-alpha and ribavirin, to treat HCV infection.
Keywords:	Microbiology;Pharmacology & Pharmacy
Date:	2005-10
Relation:	Antimicrobial Agents and Chemotherapy. 2005 Oct;49(10):4197-4202.
Link to:	http://dx.doi.org/10.1128/AAC.49.10.4197-4202.2005
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0066-4804&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000232244700028
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=25844448779
Appears in Collections:	[John Tsu-An Hsu] Periodical Articles

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