國家衛生研究院 NHRI:Item 3990099045/1160

NHRI > Institute of Biotechnology and Pharmaceutical Research > John Tsu-An Hsu > Periodical Articles > Item 3990099045/1160

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/1160

Title:	Inhibition of SARS-CoV 3C-like protease activity by theaflavin-3,3 '- digallate (TF3)
Authors:	Chen, CN;Lin, CPC;Huang, KK;Chen, WC;Hsieh, HP;Liang, PH;Hsu, JTA
Contributors:	Division of Biotechnology and Pharmaceutical Research
Abstract:	SARS-CoV is the causative agent of severe acute respiratory syndrome (SARS). The virally encoded 3C-like protease (3CL(Pro)) has been presumed critical for the viral replication of SARS-CoV in infected host cells. In this study, we screened a natural product library consisting of 720 compounds for inhibitory activity against 3CLPro. Two compounds in the library were found to be inhibitive: tannic acid (IC50 = 3 mu M) and 3-isotheaflavin-3-gallate (TF2B) (IC50 = 7 mu M). These two compounds belong to a group of natural polyphenols found in tea. We further investigated the 3CL(Pro)-inhibitory activity of extracts from several different types of teas, including green tea, oolong tea, Puer tea and black tea. Our results indicated that extracts from Puer and black tea were more potent than that from green or oolong teas in their inhibitory activities against 3CLPro. Several other known compositions in teas were also evaluated for their activities in inhibiting 3CLPro. We found that caffeine, (-)-epigallocatechin gallte (EGCg), epicatechin (EC), theophylline (TP), catechin (C), epicatechin gallate (ECg) and epigallocatechin (EGC) did not inhibit 3CLPro activity. Only theaflavin-3,3'-digallate (TF3) was found to be a 3CLPro inhibitor. This study has resulted in the identification of new compounds that are effective 3CLPro inhibitors.
Keywords:	Integrative & Complementary Medicine
Date:	2005
Relation:	Evidence-based Complementary and Alternative Medicine. 2005;2:209-215.
Link to:	http://dx.doi.org/10.1093/ecam/neh081
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000232027400012
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=24344464802
Appears in Collections:	[John Tsu-An Hsu] Periodical Articles [Hsing-Pang Hsieh] Periodical Articles

Files in This Item:

File	Description	Size	Format
000232027400012.pdf		128Kb	Adobe PDF	408	View/Open

Loading...