國家衛生研究院 NHRI:Item 3990099045/1157
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 12145/12927 (94%)
造访人次 : 859510      在线人数 : 612
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于NHRI ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/1157


    题名: Biosynthesis of human-type N-glycans in heterollogous systems
    作者: Betenbaugh, MJ;Tomiya, N;Narang, S;Hsu, JTA;Lee, YC
    贡献者: Division of Biotechnology and Pharmaceutical Research
    摘要: Insects, yeasts and plants generate widely different N-glycans, the structures of which differ significantly from those produced by mammals. The processing of the initial GlC(2)Man(9)GlcNAc(2) oligosaccharide to Man(8)GlcNAc(2) in the endoplasmic reticulum shows significant similarities among these species and with mammals, whereas very different processing events occur in the Golgi compartments. For example, yeasts can add 50 or even more Man residues to Man(8-9)GlcNAc(2), whereas insect cells typically remove most or all Man residues to generate paucimannosidic Man(3-1)GIcNAc(2) N-glycans. Plant cells also remove Man residues to yield Man(4-5)GlcNAc(2), with occasional complex GlcNAc or Gal modifications, but often add potentially allergenic beta(1,2)-linked Xyl and, together with insect cells, core alpha(1,3)-linked Fuc residues. However, genomic efforts, such as expression of exogenous glycosyltransferases, have revealed more complex processing capabilities in these hosts that are not usually observed in native cell lines. In addition, metabolic engineering efforts undertaken to modify insect, yeast and plant N-glycan processing pathways have yielded sialylated complex-type N-glycans in insect cells, and galactosylated N-glycans in yeasts and plants, indicating that cell lines can be engineered to produce mammalian-like glycoproteins of potential therapeutic value.
    关键词: Biochemistry & Molecular Biology;Cell Biology
    日期: 2004-10
    關聯: Current Opinion in Structural Biology. 2004 Oct;14(5):601-606.
    Link to: http://dx.doi.org/10.1016/j.sbi.2004.09.001
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0959-440X&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000224718800013
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=4744363988
    显示于类别:[徐祖安] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    000224718800013.pdf144KbAdobe PDF597检视/开启


    在NHRI中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈