國家衛生研究院 NHRI:Item 3990099045/1147
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 12145/12927 (94%)
造访人次 : 856841      在线人数 : 935
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于NHRI ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/1147


    题名: Combined use of GAP and AOX1 promoter to enhance the expression of human granulocyte-macrophage colony-stimulating factor in Pichia pastoris
    作者: Wu, JM;Lin, JC;Chieng, LL;Lee, CK;Hsu, TA
    贡献者: Division of Biotechnology and Pharmaceutical Research
    摘要: Glyceraldehydes-3-phosphate dehydrogenase (GAP) promoter provides a Strong constitutive expression of heterologous proteins at a level comparable to that seen with the methanol-induced alcohol oxidase (AOXI) promoter in Pichia pastoris. In the present study, strain GS115/G was created to constitutively express secreted human granulocyte-macrophage colony-stimulating factor (hGM-CSF) using GAP-based expression system. With addition of 1% casamino acids, the cultivation of GS115/G resulted in ca. 20% higher cells concentration and ca. 30% higher level of secreted protein concentration in YPD medium, The secreted proteins consisted of an unglycosylated and glycosylated recombinant hGM-CSF with different extent of glycosylation. Instead of using GAP promoter alone in strain GS115/G, the strain GS115/GA that could constitutively and inducibly express recombinant hGM-CSF was generated by transforming host strain GS115/G with AOXI promoter-controlled hGM-CSF expression cassette. In the presence of methanol, the strain GS115/GA expressed recombinant hGM-CSF at a level about two-fold higher than that constitutively expressed by GS115/G. (C) 2003 Elsevier Inc. All rights reserved.
    关键词: Biotechnology & Applied Microbiology
    日期: 2003-09-10
    關聯: Enzyme and Microbial Technology. 2003 Sep;33(4):453-459.
    Link to: http://dx.doi.org/10.1016/S0141-0229(03)00147-9
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0141-0229&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000185325800018
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0042932426
    显示于类别:[徐祖安] 會議論文/會議摘要

    文件中的档案:

    档案 描述 大小格式浏览次数
    000185325800018.pdf291KbAdobe PDF1002检视/开启


    在NHRI中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈