國家衛生研究院 NHRI:Item 3990099045/1144
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    题名: Expression of the gene coding for bacterial hemoglobin improves beta-galactosidase production in a recombinant Pichia pastoris
    作者: Wu, JM;Hsu, TA;Lee, CK
    贡献者: Division of Biotechnology and Pharmaceutical Research
    摘要: Genes coding for Vitreoscilla hemoglobin (VHb) with peroxisome targeting signal (PTS1) tag and beta-galactosidase were co-expressed in Pichia pastoris under the alcohol oxidase1 (AOX1) promoter. The expression of VHb-PTS1 had no positive effect on cell growth but significantly enhanced the whole cell beta-galactosidase activity to 4-fold higher than that of VHb-free cell in yeast extract/peptone/methanol medium under aerobic cultivation.
    关键词: Biotechnology & Applied Microbiology
    日期: 2003-09
    關聯: Biotechnology Letters. 2003 Sep;25(17):1457-1462.
    Link to: http://dx.doi.org/10.1023/A:1025072020791
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0141-5492&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000184578300013
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0041692837
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