國家衛生研究院 NHRI:Item 3990099045/1143
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    题名: Formation of enterovirus-like particle aggregates by recombinant baculoviruses co-expressing P1 and 3CD in insect cells
    作者: Hu, YC;Hsu, JTA;Huang, JH;Ho, MS;Ho, YC
    贡献者: Division of Biotechnology and Pharmaceutical Research
    摘要: The assembly of enterovirus requires the cleavage of P1 polyprotein by protease 3CD into individual structural proteins. Two recombinant baculoviruses were constructed to encode P1 and 3CD of enterovirus 71 (EV71), respectively. The expressed 3CD successfully cleaved P1 in vitro and in vivo. Also, the co-infection in insect cells resulted in crystalline virus-like particle structures morphologically resembling the authentic EV71 aggregates, which are reported for the first time.
    关键词: Biotechnology & Applied Microbiology
    日期: 2003-06
    關聯: Biotechnology Letters. 2003 Jun;25(12):919-925.
    Link to: http://dx.doi.org/10.1023/A:1024071514438
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0141-5492&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000183321200001
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0038756594
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