國家衛生研究院 NHRI:Item 3990099045/1141
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    題名: The 3C protease activity of enterovirus 71 induces human neural cell apoptosis
    作者: Li, ML;Hsu, TA;Chen, TC;Chang, SC;Lee, JC;Chen, CC;Stollar, V;Shih, SR
    貢獻者: Division of Biotechnology and Pharmaceutical Research
    摘要: The human glioblastoma SF268 cell line was used to investigate the induction of apoptosis by the 3C protease of enterovirus 71 (EV71). Transient expression in these cells of the wild-type 3C protein encoded by EV71 induced morphological alterations typical of apoptosis, including generation of apoptotic bodies. Degradation of cellular DNA in nucleosomes was also observed. When two of the amino acids in the catalytic motif of 3C were changed by mutagenesis, the 3C protein not only lost its proteolytic activity, but also its ability to induce apoptosis in the SF268 cells, Twenty-four hours after 3C transfection, poly(ADP-ribose) polymerase, a DNA repair enzyme, was cleaved, indicating that caspases were activated by the expression of EV71 3C. The 3C-induced apoptosis was blocked by the caspase inhibitors DEVD-fmk and VAD-fmk. Our findings suggest that the proteolytic activity of 3C triggers apoptosis in the SF268 cells through a mechanism involving caspase activation and that this apoptotic pathway may play an important role in the pathogenesis of EV71 infection. (C) 2002 Elsevier Science (USA).
    關鍵詞: Virology
    日期: 2002-02-15
    關聯: Virology. 2002 Feb;293(2):386-395.
    Link to: http://dx.doi.org/10.1006/viro.2001.1310
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0042-6822&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000174378100021
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0036057766
    顯示於類別:[徐祖安] 期刊論文

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