國家衛生研究院 NHRI:Item 3990099045/11279

NHRI > National Institute of Infectious Diseases and Vaccinology > Hsiu-Jung Lo > Conference Papers/Meeting Abstract > Item 3990099045/11279

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/11279

Title:	A simple and noninvasive zebrafish egg infection model for studying pathogenic yeasts
Authors:	Lo, HJ;Chen, YZ;You, MS
Contributors:	National Institute of Infectious Diseases and Vaccinology;Institute of Molecular and Genomic Medicine
Abstract:	Objective: The prevalence of fungal infections increases signiﬁcantly in the past two decades. Major infections (∼70%) are caused by Candida species. Candida albicans is polymorphic yeast that grows as yeast form which can extend to hyphae or pseudohyphae form. Disseminated candidiasis is associated with 30–40% mortality in severely immunocompromised patients. We have established a simple, noninvasive zebraﬁsh egg bath infection model, deﬁned its optimal conditions, and evaluated the model with various C. albicans mutant strains. We also applied this model to study pathogenesis of other Candida spp. Methods: Wild-type zebraﬁsh (Danio rerio), aged approximately 8 -15 months were maintained in the zebraﬁsh core facility at National Health Research Institutes (NHRI). One day post-fertilization, the embryos were sterilized using 0.028% chlorine bleach containing 0.0017% sodium hypochlorite to reduce the possibility of contamination. In general, after co-incubation, non-adhered Candida cells were removed from embryos by washing with egg water 3 times. Embryos incubated in 1 mL of egg water in 24-well plates at 30◦ C were imaged daily under an inverted microscope, with a beating heart used to indicate viability. Results: The deletion of SAP6 did not have signiﬁcant effect on the virulence. By contrast, the deletion of BCR1, CPH1, EFG1, or TEC1 signiﬁcantly reduced the virulence under current conditions. Furthermore, all embryos survived when co-incubated with bcr1/bcr1, cph1/cph1 efg1/efg1, efg1/efg1, or tec1/tec1 mutant cells. Conclusion: The results indicated that our novel zebraﬁsh model is time-saving and cost effective. Recently, we extended our novel zebraﬁsh egg infection model to study the pathogenesis of other common Candida species, including C. glabrata and C. parapsilosis.
Date:	2018-06
Relation:	Medical Mycology. 2018 Jun;56(Suppl. 2):S68.
Link to:	https://doi.org/10.1093/mmy/myy036
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1369-3786&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000434852700374
Appears in Collections:	[Hsiu-Jung Lo] Conference Papers/Meeting Abstract [Others] Conference Papers/Meeting Abstract

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