國家衛生研究院 NHRI:Item 3990099045/11153

NHRI > National Institute of Infectious Diseases and Vaccinology > Joe Yen-Hung Chow > Periodical Articles > Item 3990099045/11153

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/11153

Title:	Mutations in VP1 and 5′-UTR affect enterovirus 71 virulence
Authors:	CK, Chang;SR, Wu;YC, Chen;KJ, Lee;NH, Chung;YJ, Lu;SL, Yu;CC, Liu;YH, Chow
Contributors:	Division of Vaccine Research and Development
Abstract:	Enterovirus 71 (EV71) is a major cause of hand, foot and mouth disease (HFMD). The current EV71 propagating in Vero (EV-V) or sub-passaged in RD (EV-R) cells was used as a pathogen. Interestingly, EV-R exhibited differential virulence; challenging human scavenger receptor class B2-expressing (hSCARB2-Tg) mice with EV71 revealed that EV-V was more virulent than EV-R: 100% of mice that received lethal amounts of EV-V died, while all the mice that received EV-R survived. Severe pathogenesis correlated with viral burdens and proinflammatory cytokine levels were observed in EV-V-challenged mice, but controversy in EV-R-challenged mice. Consensus sequence analysis revealed EV-R rapidly acquired complete mutations at E145G and S241L and partial mutations at V146I of VP1, and acquired a T to C substitution at nucleotide 494 of the 5'-UTR. EV-R exhibited higher binding affinity for another EV71 receptor, human P-selectin glycoprotein ligand-1 (hPSGL-1), than EV-V. Both EV71s exhibited no significant difference in binding to hSCARB2. The molecular modelling indicate that these mutations might influence EV71 engagement with PSGL-1 and in vivo virulence.
Date:	2018-04-27
Relation:	Scientific Reports. 2018 Apr 27;8(1):Article number 6688.
Link to:	http://dx.doi.org/10.1038/s41598-018-25091-7
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2045-2322&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000431003700014
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85046141566
Appears in Collections:	[Joe Yen-Hung Chow] Periodical Articles [Chia-Chyi Liu] Periodical Articles

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