國家衛生研究院 NHRI:Item 3990099045/11054
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    題名: Human urine extract cell differentiation agent 2 protects PC12 cells from serum deprivation-induced apoptosis accompanied with priming of extracellular signal-regulated kinase activation and differentiation induction
    作者: Yao, CJ;Chuang, SE;Yang, YY;Lai, GM
    貢獻者: National Institute of Cancer Research
    摘要: OBJECTIVE: To investigate the potential neuroprotective effect of human urine extract cell differentiation agent 2 (CDA-2) by the model of serum deprivation-induced apoptosis of PC12 cells and study the underlying molecular mechanisms. METHODS: Apoptosis of PC12 cells was induced by serum deprivation. CDA-2 at doses of 0.5-4 mg/mL was used to treat the serum-deprived PC12 cells. The cellular viability was measured by sulforhodamine B binding assay and the cell apoptosis was determined by flow cytometer. Western blot was used to analyze the expression of differentiation markers and activity of extracellular signal-regulated kinase (ERK). The cellular morphology was examined under an inverted microscope. RESULTS: CDA-2 inhibited apoptotic cell death of serum-deprived PC12 cells in a dose-dependent manner. Expression of low- and mid-sized neurofilaments was observed in serum-deprived PC12 cells treated with CDA-2 or nerve growth factor (NGF). However, CDA-2 did not induce proliferation of these cells like NGF. The morphology of CDA-2 treated cells was changed from rounded to neuron-like flat polygonal shape in contrast to the extensive neurite outgrowth induced by NGF. CDA-2 transiently induced the phosphorylation of ERK in serum deprived-PC12 cells and the expression of neurofilaments induced by CDA-2 was attenuated by mitogen-activated protein/extracellular signal-regulated kinase kinase (MEK) inhibitor PD98059. CONCLUSIONS: Human urine extract CDA-2 showed a potential neuroprotective activity which may correlate with ERK activation and differentiation induction.
    日期: 2018-02-17
    關聯: Chinese Journal of Integrative Medicine. 2018 Feb 17;Article in Press.
    Link to: http://dx.doi.org/10.1007/s11655-018-2986-1
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1672-0415&DestApp=IC2JCR
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85042140227
    顯示於類別:[賴基銘(2004-2008)] 期刊論文
    [莊雙恩] 期刊論文

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