國家衛生研究院 NHRI:Item 3990099045/11001

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Title:	Identification of immunoreactive peptides of toxins to simultaneously assess the neutralization potency of antivenoms against neurotoxicity and cytotoxicity of naja atra venom
Authors:	Liu, BS;Wu, WG;Lin, MH;Li, CH;Jiang, BR;Wu, SC;Leng, CH;Sung, WC
Contributors:	National Institute of Infectious Diseases and Vaccinology
Abstract:	Assessing the neutralization capability of nonlethal but medically relevant toxins in venom has been a challenging task. Nowadays, neutralization efficacy is evaluated based simply on the survival rates of animals injected with antivenom together with a predefined dose of venom, which can determine potency against neurotoxicity but not validate the capability to neutralize cytotoxin-induced complications. In this study, a high correlation with in-vivo and in-vitro neutralization assays was established using the immunoreactive peptides identified from short-chain neurotoxin and cytotoxin A3. These peptides contain conserved residues associated with toxin activities and a competition assay indicated that these peptides could specifically block the antibody binding to toxin and affect the neutralization potency of antivenom. Moreover, the titers of peptide-specific antibody in antivenoms or mouse antisera were determined by enzyme-linked immunosorbent assay (ELISA) simultaneously, and the results indicated that Taiwanese bivalent antivenom (BAV) and Vietnamese snake antivenom-Naja (SAV-Naja) exhibited superior neutralization potency against the lethal effect of short-chain neurotoxin (sNTX) and cytotoxicity of cardiotoxin/cytotoxin (CTX), respectively. Thus, the reported peptide ELISA shows not only its potential for antivenom prequalification use, but also its capability of justifying the cross-neutralization potency of antivenoms against Naja atra venom toxicity.
Date:	2018-12-25
Relation:	Toxins. 2018 Dec 25;10(1):Article number 10.
Link to:	http://dx.doi.org/10.3390/toxins10010010
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2072-6651&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000424096500010
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85039746371
Appears in Collections:	[Wang-Chou Sung] Periodical Articles [Chih-Hsiang Leng] Periodical Articles

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