國家衛生研究院 NHRI:Item 3990099045/10792
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    題名: Rapid measurement of fluorescence lifetimes using SiPM detection and waveform sampling
    其他題名: Note: Rapid measurement of fluorescence lifetimes using SiPM detection and waveform sampling
    作者: Tsai, HM;Souris, JS;Kim, HJ;Cheng, SH;Chen, L;Lo, LW;Chen, CT;Kao, CM
    貢獻者: Institute of Biomedical Engineering and Nanomedicine
    摘要: In fluorescence spectroscopy and imaging, fluorescence lifetime measurement-assessing the average time fluorophores spend in their excited state before returning to their ground state-offers a number of advantages over quantifying fluorescence intensities that include resistance to photo-bleaching and independence from fluorophore concentration, excitation intensity, and measurement methodology. Despite growing interest, fluorescence lifetime techniques frequently mandate relatively complex instrumentation, slow data acquisition rates, and significant data analyses. In this work, we demonstrate the feasibility of measuring fluorescence lifetimes using off-the-shelf analog silicon photomultipliers and switched-capacitor array waveform sampling techniques, with precision matching that of much larger and more elaborate commercial instruments.
    日期: 2017-09
    關聯: Review of Scientific Instruments. 2017 Sep;88(9):Article number 096107.
    Link to: http://dx.doi.org/10.1063/1.5003413
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0034-6748&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000412108700085
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