Paper-based devices chemically functionalized with capturing molecules enable the isolation and characterization of extracellular vesicles (EVs) from samples of limited amount. Here, we describe the isolation of EV subpopulations from human serum samples. The morphology, content, and amount of captured EVs can be assessed using scanning electron microscopy (SEM ), transcriptome analysis, and paper-based enzyme-linked immunosorbent assays (pELISA), respectively. A colorimetric readout can be detected from 10 muL serum within 10 min.
Date:
2017-08-22
Relation:
Methods in Molecular Biology. 2017 Aug 22;1660:43-54.
