國家衛生研究院 NHRI:Item 3990099045/10663
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    题名: Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform
    作者: Gopinathan, P;Hung, LY;Wang, CH;Chiang, NJ;Wang, YC;Shan, YS;Lee, GB
    贡献者: National Institute of Cancer Research
    摘要: An integrated microfluidic system capable of automatically identifying aptamers specific to cholangiocarcinoma (CCA) cells was developed herein. The developed system was capable of performing cell-based systematic evolution of ligands via an exponential enrichment (Cell-SELEX) process on-chip, and only six rounds of Cell- SELEX were required to identify high specificity aptamers; this represents a significant improvement in speed over conventional SELEX, in which 15-20 rounds are typically required. Using the microfluidic chip developed, three aptamers specific to CCA cells (one for SNU-478 cells and two for HuCCT-1 cells) were successfully screened. This automated system could be modified to uncover aptamer probes against other cancer cells, thereby allowing for earlier diagnosis and consequently a potentially improved prognosis.
    日期: 2017-07-01
    關聯: Biomicrofluidics. 2017 Jul 1;11(4):Article number 044101.
    Link to: http://dx.doi.org/10.1063/1.4991005
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1932-1058&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000407353100002
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85021855446
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