國家衛生研究院 NHRI:Item 3990099045/10563
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    题名: Fruits as the vehicle of drug resistant pathogenic yeasts
    作者: Lo, HJ;Tsai, SH;Chu, WL;Chen, YZ;Zhou, ZL;Chen, HF;Lee, CF;Yang, YL
    贡献者: Division of Infectious Diseases
    摘要: OBJECTIVE: We investigated the diversity and drug susceptibility of pathogenic yeasts on fruit surfaces. METHOD: Fruits were purchased from supermarkets and washed with buffer. The pellets were re-suspended in medium after centrifugation. The cell suspensions were plated onto CHROMagar Candida medium. Yeasts were identified by ribosomal DNA sequencing and their drug susceptibilities were determined by broth microdilution assay. RESULTS: Of 184 isolates, comprised of 55 species, from 22 different types of fruits, 29 species, including Candida famata, Candida fermentati, Candida guilliermondii, Candida intermedia, Candida krusei, Candida orthopsilosis, Candida parapsilosis, Candida pelliculosa, Candida tropicalis, and others have been reported to cause diseases in humans. In addition to C. krusei, intrinsically resistant to fluconazole, all Rhodotorula and Rhodosporidium species were resistant to fluconazole. One each of C. tropicalis isolate was belonged to diploid sequence type (DST)149 and DST225, genotypes also detected in isolates from humans. Furthermore, the DST225 isolate was less susceptible to azole drugs. The susceptibilities to azole drugs for clinical and agricultural usage were associated to each other. CONCLUSION: It is important to be aware of the existence of pathogenic yeasts, especially drug resistant ones, on the fruit surfaces, a potential route for pathogenic yeasts to be transmitted to humans.
    日期: 2017-08
    關聯: Journal of Infection. 2017 Aug;75(3):254–262.
    Link to: http://dx.doi.org/10.1016/j.jinf.2017.06.005
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0163-4453&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000408022800009
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85021436644
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