國家衛生研究院 NHRI:Item 3990099045/10561
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/10561


    Title: Extracellular matrix protein laminin enhances mesenchymal stem cell (MSC) paracrine function through alphavbeta3/CD61 integrin to reduce cardiomyocyte apoptosis
    Other Titles: Extracellular matrix protein laminin enhances mesenchymal stem cell (MSC) paracrine function through αvβ3/CD61 integrin to reduce cardiomyocyte apoptosis
    Authors: Peng, KY;Liu, YH;Li, YW;Yen, BL;Yen, ML
    Contributors: Institute of Cellular and Systems Medicine
    Abstract: Myocardial ischaemia (MI) results in extensive cardiomyocyte death and reactive oxygen species (ROS)-induced damage in an organ with little or no regenerative capacity. Although the use of adult bone marrow mesenchymal stem cells (BMMSCs) has been proposed as a treatment option, the high cell numbers required for clinical use are difficult to achieve with this source of MSCs, and animal studies have produced inconsistent data. We recently demonstrated in small and large animal models of acute MI that the application of human term placenta-derived multipotent cells (PDMCs), a foetal-stage MSC, resulted in reversal of cardiac injury with therapeutic efficacy. However, the mechanisms involved are unclear, making it difficult to strategize for therapeutic improvements. We found that PDMCs significantly reduced cardiomyocyte apoptosis and ROS production through the paracrine factors GRO-alpha, HGF and IL-8. Moreover, culturing PDMCs on plates coated with laminin, an extracellular matrix (ECM) protein, resulted in significantly enhanced secretion of all three paracrine factors, which further reduced cardiomyocyte apoptosis. The enhancement of PDMC paracrine function by laminin was mediated through alphavbeta3 integrin, with involvement of the signalling pathways of JNK, for GRO-alpha and IL-8 secretion, and PI3K/AKT, for HGF secretion. Our results demonstrated the utility of PDMC therapy to reduce cardiomyocyte apoptosis through modulation of ECM proteins in in vitro culture systems as a strategy to enhance the therapeutic functions of stem cells.
    Date: 2017-08
    Relation: Journal of Cellular and Molecular Medicine. 2017 Aug;21(8):1572-1583.
    Link to: http://dx.doi.org/10.1111/jcmm.13087
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1582-1838&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000406833000013
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85020390961
    Appears in Collections:[Betty Lin-Ju Yen] Periodical Articles

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