國家衛生研究院 NHRI:Item 3990099045/10543

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Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/10543

Title:	High fat diet induces mitochondria stress and impairs myelin structure in rat hypothalamus
Authors:	Huang, HT;Hsien, HH;Wu, HT;Tsai, SF;Huang, HY;Kuo, YM;Chen, PS;Yang, CS;Tzeng, SF
Contributors:	Institute of Biomedical Engineering and Nanomedicine
Abstract:	Obesity-induced central mild inflammation and metabolic control in the hypothalamus have been well documented. Gliosis, a reactive change of astrocytes and microglia, is observed in the hypothalamus of obese rodents and humans. However, the reaction of oligodendrocytes (OLGs), a CNS myelin producing glia population, in the hypothalamus of obese individuals is poorly known. Using high-fat diet (HFD)-fed mouse model, we found that Iba1+ microglia in arcuate nucleus (ARC) and median eminence (ME) in the hypothalamus of HFD-fed mice displayed an amoeboid hypertrophic form. Interestingly, we detected an increase in the protein level of IL-27, a pleiotropic cytokine that has been recently shown to inhibit the severity of experimental autoimmune encephalomyelitis. Through comparative microRNA microarray, we observed an increase in a group of microRNAs in the hypothalamus at 3 month post HFD feeding. Among these microRNAs, we confirmed that let-7f, a regulator of brain development and glucose metabolism, was increased in the hypothalamus after HFD feeding for 4 months. Moreover, the results from transmission electron microscopy (TEM) analysis indicated that fragmented or hyperfused mitochondria was observed in the hypothalamus of HFD-fed mice. The disturbed structure of myelin in the hypothalamus of HFD-fed mice was also detected by TEM. Based on these observations, we suggest that HFD-induced mild inflammation and changes in mitochondrial functions might impair metabolism homeostasis in OLGs, and cause an injury in myelin microstructure.
Date:	2017-06
Relation:	Glia. 2017 Jun;65(Suppl.):E207.
Link to:	http://dx.doi.org/10.1002/glia.23157
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0894-1491&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000403071700317
Appears in Collections:	[Chung-Shi Yang] Conference Papers/Meeting Abstract

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