國家衛生研究院 NHRI:Item 3990099045/10370
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 12145/12927 (94%)
造訪人次 : 856861      線上人數 : 945
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於NHRI ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/10370


    題名: Knocking out of SWAP-70 makes spontaneously transformed NIH3T3 cells normal
    作者: Fukui, Y
    貢獻者: Institute of Cellular and Systems Medicine
    摘要: Recently we showed that SWAP‐70 knocked out mouse embryo fibroblasts (MEFs) did not transform for a long time and adding back of SWAP‐70 restored transforming ability. It is well known that NIH3T3 cells easily transform spontaneously. This year we used spontaneously transformed NIH3T3 cells. Using a kit, the SWAP‐70 gene was knocked out. After knocking out of the SWAP‐70 gene, growth rate of the cells was diminished and contact inhibition ability was restored. They required high concentration of serum to grow whereas the spontaneously transformed cells grew in the medium with low concentration of serum. They became resistant to nutrient depletion when the spontaneously transformed cells were sensitive to nutrient depletion. The parental the spontaneously transformed NIH3T3 cells grew in soft agar but the SWAP‐70 knocked out cells did not. Finally, the spontaneously transformed cells made tumors in nude mice but the SWAP‐70 knocked out cells did not. Taken together, SWAP‐70 is required for spontaneous transformation of rodent cells. It is interesting to see what happens when the SWAP‐70 gene of human tumors is disrupted.
    日期: 2015
    關聯: Molecular Biology of the Cell. 2015;26:Meeting Abstract P247.
    Link to: http://www.molbiolcell.org/
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1059-1524&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000209928401274
    顯示於類別:[福井泰久(2010-2016)] 會議論文/會議摘要

    文件中的檔案:

    沒有與此文件相關的檔案.



    在NHRI中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋