國家衛生研究院 NHRI:Item 3990099045/10092
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/10092


    Title: Neural Stem Cells promote nerve regeneration through IL12-induced schwann cell differentiation
    Authors: Lee, DC;Chen, JH;Hsu, TY;Chang, LH;Chang, H;Chi, YH;Chiu, IM
    Contributors: Institute of Cellular and Systems Medicine;Institute of Biomedical Engineering and Nanomedicine
    Abstract: Regeneration of injured peripheral nerves is a slow, complicated process that could be improved by implantation of neural stem cells (NSCs) or nerve conduit. Implantation of NSCs along with conduits promotes the regeneration of damaged nerve, likely because (i) conduit supports and guides axonal growth from one nerve stump to the other, while preventing fibrous tissue ingrowth and retaining neurotrophic factors; and (ii) implanted NSCs differentiate into Schwann cells and maintain a growth factor enriched microenvironment, which promotes nerve regeneration. In this study, we identified IL12p80 (homodimer of IL12p40) in the cell extracts of implanted nerve conduit combined with NSCs by using protein antibody array and Western blotting. Levels of IL12p80 in these conduits are 1.6-fold higher than those in conduits without NSCs. In the sciatic nerve injury mouse model, implantation of NSCs combined with nerve conduit and IL12p80 improves motor recovery and increases the diameter up to 4.5-fold, at the medial site of the regenerated nerve. In vitro study further revealed that IL12p80 stimulates the Schwann cell differentiation of mouse NSCs through the phosphorylation of signal transducer and activator of transcription 3 (Stat3). These results suggest that IL12p80 can trigger Schwann cell differentiation of mouse NSCs through Stat3 phosphorylation and enhance the functional recovery and the diameter of regenerated nerves in a mouse sciatic nerve injury model.
    Date: 2017-03
    Relation: Molecular and Cellular Neurosciences. 2017 Mar;79:1-11.
    Link to: http://dx.doi.org/10.1016/j.mcn.2016.11.007
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1044-7431&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000394730400001
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85008233253
    Appears in Collections:[Ing-Ming Chiu] Periodical Articles
    [Ya-Hui Chi] Periodical Articles
    [Hsu Chang(2004-2017)] Periodical Articles

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