國家衛生研究院 NHRI:Item 3990099045/10043
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 12145/12927 (94%)
造访人次 : 914426      在线人数 : 1391
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于NHRI ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/10043


    题名: Toll-like receptor 2 promotes vascular calcification via the chondrogenic transdifferentiation of vascular smooth muscle cells
    作者: Lee, GL;Kuo, CC
    贡献者: Institute of Cellular and Systems Medicine
    摘要: Objective: vascular smooth muscle cell (VSMC) transformation to osteoblast/ chondrocyte Phenotype is an initiative event for arterial calcification, which is highly correlated with cardiovascular disease morbidity and mortality. Toll-like receptors (TLRs) play key roles in the development of vascular diseases, but their regulation in vascular calcification remains unclear. Methods and results: Calcification assays by Alizarin red S staining revealed that TLR2 agonists promoted VSMC calcification. TLR2 deficiency or inhibition of TLR2 signaling with anti-TLR2 antibody suppressed TLR2 agonist-induced VSMC calcification and IL-6 production. Neutralizing anti-IL-6 antibodies impaired TLR2-mediated VSMC calcification, while addition of IL-6 recombinant protein promoted VSMC calcification. Additionally, ApoE-/- mice fed high-fat diet (HFD) exhibited vascular calcium accumulation and serum IL-6 elevation, which were ameliorated in Tlr2-/-ApoE-/- mice. Further studies demonstrated that TLR2 agonist time-dependently increased chondrogenic transdifferentiation transcription factors SOX9 and osterix but suppressed osteo/chondrogenic transdifferentiation transcription factor Runx2 and osteoprotegerin (OPG) in wild-type but not in Tlr2 -/- VSMCs. Furthermore, TLR2-induced transdifferentiation of wild-type VSMCs into chondrogenic cells was suppressed by both of neutralizing anti-IL6 antibodies and OPG recombinant protein. Conclusions: Our results suggest that upon ligand binding, TLR2 promotes chondrogenic transdifferentiation of VSMC via modulating expression of IL-6 and OPG production, SOX9 and osterix. The chondrogenic VSMCs in turn contributes to vascular calcification during the pathogenesis of atherosclerosis.
    日期: 2016-08
    關聯: European Journal of Immunology. 2016 Aug;46(Suppl. 1):371.
    Link to: http://dx.doi.org/10.1002/eji.201670200
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0014-2980&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000383610400745
    显示于类别:[郭呈欽] 會議論文/會議摘要

    文件中的档案:

    档案 描述 大小格式浏览次数
    ISI000383610400745.pdf188KbAdobe PDF398检视/开启


    在NHRI中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈