國家衛生研究院 NHRI:Item 3990099045/10038

NHRI > National Institute of Infectious Diseases and Vaccinology > Others > Conference Papers/Meeting Abstract > Item 3990099045/10038

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/10038

Title:	Validation and application of a commercial quantitative real-time Reverse transcriptase-PCR assay in investigation of a large dengue virus outbreak in southern Taiwan
Authors:	Tsai, HP;Tsai, YY;Lin, IT;Kuo, PH;Chang, KC;Chen, JC;Ko, WC;Wang, JR
Contributors:	Division of Infectious Diseases
Abstract:	Background: Accurate, rapid, and early diagnosis of dengue virus (DENV) infections is essential for optimal clinical care. Here, we evaluated the efficacy of the quantitative real-time PCR (qRT-PCR)-LightMix dengue virus EC kit for DENV detection using samples from a dengue outbreak in Taiwan in 2015. Methods: Sera from patients with suspected DENV infection were analyzed and compared using the LightMix kit, a Dengue NS1 Ag + Ab Combo kit for detection of NS1 antigen and DENV-specific IgM and IgG antibodies, and an “in-house” qualitative DENV-specific RT-PCR assay. Results: A total of 8,989, 8,954, and 1581 samples were subjected to NS1 antigen detection, IgM and IgG detection, and LightMix assays, respectively. The LightMix assay yielded a linear curve for viral loads (VL) between 102 and 106 copies/reaction, and the minimum detection limits for DENV serotype 1 (DENV1) and DENV2, DENV3, and DENV4 were 1, 10, and 100 focus forming units (FFU)/mL, respectively. There was 88.9% concordance between the results obtained using the NS1 antigen combo kit and by LightMix analysis, and the diagnostic sensitivity and specificity of the two methods were 89.4 and 100%, and 84.7 and 100%, respectively. Notably, fatal cases were attributed to DENV2 infection, and 79.5% (27/34) of these cases occurred in patients ≥ 71 years of age. Among these older patients, 82.3% (14/17) were NS1/IgM/IgG (+/-/-), exhibiting VLs between 106–109 copies/mL, which was markedly higher than the rate observed in the other age groups. Conclusions: The LightMix assay was effective for early diagnosis of DENV infection. Our data indicate that high VLs during primary infection in elderly patients may be a positive predictor for severe illness, and may contribute to high mortality rates.
Date:	2016-10
Relation:	PLoS Neglected Tropical Diseases. 2016 Oct;10(10):Article number e0005036.
Link to:	http://dx.doi.org/10.1371/journal.pntd.0005036
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1935-2735&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000386676200026
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84994316527
Appears in Collections:	[Others] Conference Papers/Meeting Abstract

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