國家衛生研究院 NHRI:Item 3990099045/3940
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    题名: Introduction of a strong temperature-sensitive phenotype into enterovirus 71 by altering an amino acid of virus 3D polymerase
    作者: Kung, YH;Huang, SW;Kuo, PH;Kiang, D;Ho, MS;Liu, CC;Yu, CK;Su, IJ;Wang, JR
    贡献者: Division of Infectious Diseases
    摘要: In 1998, an enterovirus 71 (EV71) epidemic in Taiwan resulted in 78 deaths; however, the molecular basis of EV71 pathogenicity remains poorly understood. Comparison of the deduced amino acid sequences in 3D polymerases of EV71clinical isolates showed the T251V or T251I substitution from 1986 and 1998 outbreaks. An EV71 replicon system showed that introducing an I251T mutation did not affect luciferase activities at 35?°C when compared with wild type; however, lower luciferase activities were observed when they were incubated at 39.5?°C. In addition, the I251T mutation in the EV71 infectious clone not only reduced viral replication at 39.5?°C in vitro but also decreased the virulence of the mouse adaptive strain MP4 in neonatal mice in an i.p. infection model. Therefore, these results suggested that the threonine at position 251 results in a temperature sensitivity phenotype of EV71 which may contribute to the attenuation of circulating strains.
    日期: 2010-01-05
    關聯: Virology. 2010 Jan 5;396(1):1-9.
    Link to: http://dx.doi.org/10.1016/j.virol.2009.10.017
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0042-6822&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000272647600001
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=70649113050
    显示于类别:[蘇益仁(2002-2015)] 期刊論文

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